CUSTOM ITEM: HPLC Column, Bidentate C8 300, 5um, 2.1mm ID x 100mm Length, 300A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40008‑10P‑2‑3M Cogent Bidentate C8™ 300 HPLC Column is a custom‑manufactured, wide‑pore reversed‑phase column specifically designed for large molecular‑weight analytes, including proteins, peptides, polysaccharides, and other macromolecules. Built on Cogent TYPE‑C™ silica hydride technology, this column delivers excellent selectivity, faster equilibration, and extremely robust column lifetime compared with conventional 300 Å silica‑based C8 columns.
The stationary phase utilizes an aliphatic C8 hydrocarbon chain bonded via direct silicon–carbon bonds to a silica hydride surface. This chemistry produces a phase that is more hydrophobic than traditional wide‑pore C8 columns, yet appropriately balanced for macromolecules—avoiding the excessive retention often observed with C18 while providing stronger interactions than standard C8 phases. The 300 Å pore structure ensures efficient access and mass transfer for large biomolecules, improving peak shape and resolution.
Unlike typical 300 Å C8 materials, the Bidentate C8™ 300 can retain compounds that often do not retain on standard silica phases and can also retain select hydrophilic macromolecules, particularly when operated in HILIC‑like modes. The column is stable at very low pH (below pH 2) without degradation, enabling aggressive methods for biomolecule analysis.
The 2.1 mm internal diameter supports low‑flow operation, reduced solvent consumption, and enhanced MS sensitivity, while the 100 mm length provides increased resolving power relative to 50 mm formats. This configuration is frequently chosen for LC‑MS workflows requiring higher resolution for large molecules.
Why Choose Bidentate C8™ 300 for Wide‑Pore Applications
This column is typically selected when:
Analytes are large biomolecules that require 300 Å pores
Conventional wide‑pore C8 columns lack sufficient retention or stability
Excessive retention on C18 must be avoided for macromolecules
Low‑pH operation is required without column damage
Higher resolution is desired beyond short wide‑pore formats
The combination of 300 Å pores and TYPE‑C™ silica hydride chemistry provides a method window that conventional wide‑pore silica columns cannot match.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Direct Si–C bonding for exceptional chemical robustness
Reduced silanol activity and secondary interactions
Faster equilibration than conventional wide‑pore silica columns
Bidentate C8™ 300 Bonding
Enhanced hydrophobicity vs standard C8
Balanced retention for large biomolecules
Avoids excessive adsorption seen with C18
Wide‑Pore (300 Å) Architecture
Optimized for proteins, peptides, and polysaccharides
Improved mass transfer and peak shape
Supports macromolecular separations
LC‑MS‑Optimized Geometry
2.1 mm ID for low‑flow sensitivity
100 mm length for improved resolution
Requires precise low‑flow pumping and reduced‑volume flow cells
Aggressive Method Compatibility
Stable at very low pH (below 2)
Compatible with aqueous‑rich mobile phases
Supports HILIC‑like operation for select macromolecules
System Requirements
For optimal performance with this narrow‑bore, wide‑pore column, use:
A pump capable of accurate, stable low‑flow delivery
Detector flow cells designed for low internal volume
LC‑MS systems configured for biomolecular analysis
Typical Applications
LC‑MS of proteins, peptides, and polysaccharides
Biomolecule separation and characterization
Wide‑pore reversed‑phase separations
High‑resolution analysis of macromolecules
Low‑pH biomolecule methods
Alternative HILIC‑mode macromolecule analysis
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable